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| ID | Components | Size |
| RES054-C01 | Pre-coated Anti-Nuclease Antibody Microplate | 1 plate |
| RES054-C02 | Salt Active Nuclease Standard | 5 μg |
| RES054-C03 | Biotin-Anti-Nuclease Antibody | 20 μg |
| RES054-C04 | Streptavidin-HRP | 50 μL |
| RES054-C05 | 10×Washing Buffer | 50 mL |
| RES054-C06 | 2×Dilution Buffer | 50 mL |
| RES054-C07 | Substrate Solution | 12 mL |
| RES054-C08 | Stop Solution | 7 mL |
The Salt Active GENIUS™ELISA Kit was developed for the detection and quantitative determination of nuclease in samples from downstream processing where nuclease is used as a process or purification aid.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. Please use it up once you opened it.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the Nuclease as standard. The samples and standard are diluted by Dilution Buffer.
c) Add the Biotin-Anti-Nuclease Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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