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Your Position: Home > Antibody > HLA class I/W632 > HLI-CFM597

PE-Labeled Monoclonal Anti-HLA class I Antibody, Human IgG1 (W632)

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  • Source
    PE-Labeled Monoclonal Anti-HLA class I Antibody, Human IgG1 (W632) is a chimeric monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with HLA class I/W632.
  • Isotype
    Human IgG1 | Human Kappa
  • Specificity
    This product is a specific antibody specifically reacts with HLA class I/W632.
  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Application
    FACS
  • Purity

    >90% as determined by SDS-PAGE.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20 to -70°C for 12 months in lyophilized state from date of receipt;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-FACS
 HLA class I/W632 FACS

Flow cytometric analysis of Jurkat cells staining with PE-Labeled Monoclonal Anti-HLA class I Antibody, Human IgG1 (W632) (Cat. No. HLI-CFM597) at 1:50 dilution(2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) , compared with isotype control antibody. PE signal was used to evaluate the binding activity (QC tested).

  • Background
    The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains.
  • Clinical and Translational Updates

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Price(USD) : $226.00

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