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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 63.3 kDa. The protein migrates as 45-47 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human ADORA2A Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Human ADORA2A Protein, His Tag (Cat. No. ADA-H52H5) at 10 μg/mL (100 μL/well) on a Nickel Coated plate can bind Anti-ADORA2A Antibody with a linear range of 0.1-2 ng/mL (QC tested).
Anti-ADORA2A antibody-3F6-9G5 captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human ADORA2A Protein, His Tag (Cat. No. ADA-H52H5) with an affinity constant of 21.8 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).
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