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This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag
The protein has a calculated MW of 50.9 kDa. The protein migrates as 55-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human APRIL Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Human APRIL Protein, Avitag,His Tag (Cat. No. APL-H82Q8) is more than 90% and the molecular weight of this protein is around 50-70 kDa verified by SEC-MALS.
Immobilized Biotinylated Human APRIL Protein, Avitag,His Tag (Cat. No. APL-H82Q8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human BCMA, Fc Tag, premium grade (Cat. No. BC7-H5254) with a linear range of 0.1-2 ng/mL (QC tested).
Immobilized Biotinylated Human APRIL Protein, Avitag,His Tag (Cat. No. APL-H82Q8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human TACI (R119A, R122A), Fc Tag (Cat. No. TAI-H5253) with a linear range of 2-39 ng/mL (Routinely tested).
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