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| ID | Components | Size |
| RAS159-C01 | Pre-coated Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody Microplate | 1 plate |
| RAS159-C02 | Pre-Fusion glycoprotein F0 (RSV) Standard | 20 μg |
| RAS159-C03 | Biotin-Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody | 20 μg |
| RAS159-C04 | Streptavidin-HRP | 50 μL |
| RAS159-C05 | 10xWashing Buffer | 50 mL |
| RAS159-C06 | 2xDilution Buffer | 50 mL |
| RAS159-C07 | Substrate Solution | 12 mL |
| RAS159-C08 | Stop Solution | 7 mL |
A rapid and effective assay kit detecting the levels of HRSV Pre-Fusion glycoprotein F0 is urgently needed to accelerate the development of RSV vaccines.
This kit is developed for Specific quantitative detection of HRSV Pre-Fusion glycoprotein F0 in samples.
This kit can specifically identify HRSV (A) and HRSV (B) Pre-Fusion glycoprotein F0 by testing.
It is for research use only.
Your experiment will include 6 simple steps:
a) All reagents were returned to room temperature(20℃-25℃) before use.
b) Add your sample to the plate, take the Pre-Fusion glycoprotein F0 (RSV) as standard sample. The samples and standard sample are diluted by Dilution Buffer.
c) Wash the plate and add the Biotin-Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the diluted Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
Detection of Pre-Fusion glycoprotein F0 (RSV) by sandwich-ELISA Assay.
Immobilized Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody can bind Pre-Fusion glycoprotein F0 (RSV). Detection was performed using Biotin-Pre-Fusion glycoprotein F0 (RSV) Antibody with sensitivity of < 46.9 pg/mL (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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