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Your Position: Home > Protein > Pyrophosphatase > PYE-T5143

Thermostable Inorganic Pyrophosphatase (2 U/μl)

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  • Product Details
    This product is a purified recombinant inorganic pyrophosphatase (PPase) in Escherichia coli.
    Inorganic pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate:
    P2O7-4+H2O+PPase→2HPO4-2
    PPase can hydrolyze inorganic pyrophosphate generated with the reaction to avoid its inhibition of the reaction system. It can be used to increase RNA yield in reverse transcription reactions. This enzyme retains 80% activity after incubation at 100°C for 4 hours.
  • Application
    1. Increase RNA yield in transcription reaction;

    2. Enhance DNA replication;

    3. Hydrolyze inorganic pyrophosphate.

  • Unit Definition
    One unit is the amount of enzyme that will generate 1 µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (at 75°C in 50 mM Tricine, pH 8.5, 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml for 10 minute).
  • Quility Control
    No endodeoxyribonucleases, exodeoxyribonucleases and Ribonucleases residues.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris,100 mM NaCl,1 mM DTT,0.1 mM EDTA,pH 8.0 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with blue ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable for up to 12 months at -20℃ from date of receipt.

  • Notes

    This product is active in a variety of reaction buffers and can usually be added directly to amplification experiments such as HDA and LAMP.

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