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Your Position: Home > Protein > Siglec-3 > CD3-H5258

Human Siglec-3 / CD33 (140-232) Protein, Llama IgG2b Fc Tag, low endotoxin (MALS verified)

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  • Synonym
    CD33,SIGLEC3,gp67
  • Source
    Human Siglec-3 (140-232), Llama IgG2b Fc Tag(CD3-H5258) is expressed from human 293 cells (HEK293). It contains AA Asp 140 - Thr 232 (Accession # P20138-1).
    Predicted N-terminus: Asp 140
  • Molecular Characterization
    Siglec-3 Structure

    This protein carries a llama IgG2b Fc tag at the C-terminus.

    The protein has a calculated MW of 37.8 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Siglec-3 SDS-PAGE

Human Siglec-3 (140-232), Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Siglec-3 MALS images

The purity of Human Siglec-3 (140-232), Llama IgG2b Fc Tag (Cat. No. CD3-H5258) is more than 85% and the molecular weight of this protein is around 77-104 kDa verified by SEC-MALS.

  • Background
    Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cD encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.
  • Clinical and Translational Updates

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