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Your Position: Home > Kits > IL-4 > EP-132

IL-4 [Biotinylated] : IL-4Rα Inhibitor Screening ELISA Kit

For research use only.

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Materials Provided
EP132-C01High-bind Plate1 plate
EP132-C02Human IL-4 R alpha30 μg
EP132-C03Human IL-420 μg
EP132-C04Human IL-4-Biotin10 μg
EP132-C05Streptavidin-HRP10 μg
EP132-C06Coating Buffer12 mL
EP132-C0710xWashing Buffer 50 mL
EP132-C08Blocking Buffer50 mL
EP132-C09Substrate Solution12 mL
EP132-C10Stop Solution7 mL
  • Background
    Interleukin-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells to Th2 cells). In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine.
  • Application
    This kit is developed for screening for inhibitors of human IL-4Rα binding to human IL-4.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new IL-4 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human IL-4 to immobilized human IL-4 R alpha in a functional ELISA assay, and employs a simple colorimetric sandwich ELISA platform.

    Your experiment will include 4 simple steps:

    a) Coat the plate with human IL-4 R alpha.

    b) Add your molecule of interest to the tests.

    c) Add human IL-4-Biotin to bind the coated human IL-4 R alpha.

    d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

    Finally, the ability of your compound to inhibit IL-4: IL-4 R alpha Rbinding will be determined by comparing OD readings among different experimental groups.

Typical Data
IL-4 [Biotinylated] : IL-4Rα Inhibitor Screening ELISA KitIL-4 [Biotinylated] : IL-4Rα Inhibitor Screening ELISA Kit (Cat. No. EP-132) ELISA bioactivity

Serial dilutions of Human IL-4(Catalog # EP132-C03) (1:1 serial dilution, from 5 μg/mL to 0.01 μg/mL (295.86-0.58 nM)) was added into IL-4 R alpha: IL-4-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

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