Acrobiosystems for English
icon_bulk_orderBulk inquiry/Quick order
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Protein > CFH > CFH-H52H3

Human CFH Protein, His Tag (MALS verified)

Order Now

  • Synonym
    AHUS1, AMBP1, ARMD4, ARMS1, CFHL3, FH, FHL1, HF, HF1, HF2, HUS
  • Source
    Human CFH Protein, His Tag(CFH-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 868 - Arg 1231 (Accession # P08603).
    Predicted N-terminus: Ile 868
  • Molecular Characterization
    CFH Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 42.7 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CFH SDS-PAGE

Human CFH Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CFH MALS images

The purity of Human CFH Protein, His Tag (Cat. No. CFH-H52H3) is more than 90% and the molecular weight of this protein is around 50-70 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CFH ELISA

Immobilized Human CFH Protein, His Tag (Cat. No. CFH-H52H3) at 10 μg/mL (100 μL/well) can bind Heparin Biotin with a linear range of 0.01-1.25 μg/mL (QC tested).

Bioactivity-SPR
 CFH SPR

Human ITGAM&ITGB2 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT2-H52W4) immobilized on CM5 Chip can bind Human CFH Protein, His Tag (Cat. No. CFH-H52H3) with an affinity constant of 4.24 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Complement Factor H (CFH) composed of 20 repetitive units of 60 amino acids, named short consensus repeats (SCR) or complement control protein modules (CCP), arranged in a continuous fashion. CFH is amongst the most abundant complement components in human blood, where the liver is the major source of CFH protein in vivo. The CFH molecule includes different interaction sites for C3b and polyanions which delineate distinct functional domains at the N- and C-termini. The C3b binding site in SCR1-4 is the only site essential for the factor I co-factor activity of factor H. Similarly, the C3b/polyanions-binding site located within SCR19-20 is the most important site for preventing alternative pathway activation on host cells. Mutations in the CFH gene are associated with severe and diverse diseases including the rare renal disorders hemolytic uremic syndrome (HUS) and membranoproliferative (MPGN) also termed dense deposit disease (DDD), as well as the more frequent retinal disease age related macular degeneration (AMD).
  • Clinical and Translational Updates

Comments (0)


ETA of in-stock products: 2 business days

Price(USD) : $368.00

Price(USD) : $2425.00

Contact Us

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message