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Your Position: Home > Other Coronavirus Proteins > Spike protein > SPN-B52H8

Bat SARS-like coronavirus RsSHC014 Spike Trimer (R668A, KA969-970PP), His Tag (MALS verified)

  • Synonym
    Spike,S protein,Spike glycoprotein,S glycoprotein
  • Source
    Bat SARS-like coronavirus RsSHC014 Spike Trimer, His Tag (SPN-B52H8) is expressed from human 293 cells (HEK293). It contains AA Tyr 15 - Pro 1196 (Accession # U5WLK5(R668A, KA969-970PP)).
    Predicted N-terminus: Tyr 15
  • Molecular Characterization
    Online(Tyr 15 - Pro 1196) U5WLK5 (R668A, KA969-970PP)

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 136.2 kDa. The protein migrates as 170-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Bat SARS-like coronavirus RsSHC014 Spike Trimer, His Tag (Cat. No. SPN-B52H8) SDS-PAGE gel

Bat SARS-like coronavirus RsSHC014 Spike Trimer, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Bat SARS-like coronavirus RsSHC014 Spike Trimer, His Tag (Cat. No. ) MALS images

The purity of Bat SARS-like coronavirus RsSHC014 Spike Trimer, His Tag (Cat. No. SPN-B52H8) is more than 85% and the molecular weight of this protein is around 483-534 kDa verified by SEC-MALS.

Bioactivity-ELISA
Bat SARS-like coronavirus RsSHC014 Spike Trimer, His TagBat SARS-like coronavirus RsSHC014 Spike Trimer, His Tag (Cat. No. SPN-B52H8) ELISA bioactivity

Immobilized Bat SARS-like coronavirus RsSHC014 Spike Trimer, His Tag (Cat. No. SPN-B52H8) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-4 ng/mL (QC tested).

  • Background
    It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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