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Your Position: Home > Membrane Protein > Claudin-9 > CL9-H5586

Human Claudin-9 / CLDN9 Protein, His,Twin-Strep Tag (active membrane protein, SPR verified)

  • Synonym
    CLDN9
  • Source
    Human Claudin-9, His,Twin-Strep Tag (CL9-H5586) is expressed from Baculovirus-Insect cells. It contains AA Ala 2 - Val 217 (Accession # O95484-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    Online(Ala 2 - Val 217) O95484-1

    This protein carries a polyhistidine tag and a twin strep tag.

    The protein has a calculated MW of 27.9 kDa. The protein migrates as 23-24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

    This product is not suitable for cell based experiments due to cytotoxicity of DDM.

    DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.

    DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.

    If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.

    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 12 months under sterile conditions.
  • Shipping
    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.
*The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.
SDS-PAGE
Human Claudin-9, His,Twin-Strep Tag (Cat. No. CL9-H5586) SDS-PAGE gel

Human Claudin-9, His,Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-Claudin 9 antibody captured on Protein G-Series S sensor chip can bind Human Claudin-9, His,Twin-Strep Tag (Cat. No. CL9-H5586) with an affinity constant of 38.8 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (QC tested).

  • Background
    Claudin-9 belongs to the claudin family. Claudins constitute integral membrane proteins responsible for solute and electrolyte permeability of the tight junction that serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Tight junctions also play a critical role in maintaining cell polarity and signal transductions. Claudin-9 creates charge specific channels in the paracellular space, plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity, is required to preserve sensory cells in the hearing organ because claudin-9-defective tight junctions fail to shield the basolateral side of hair cells from the K+-rich endolymph. Its ion barrier function is essential in the cochlea, but appears to be dispensable in other organs. Is one of the entry cofactors for hepatitis C virus; it enables HCV entry into target cells just as efficiently as CLDN1.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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