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GENIUS™ Nuclease, premium grade

GMP version GMP-NUES19 is now available for seamless transition.

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  • Features

    GENIUS™ Nuclease, premium grade is designed for preclinical stage, has more comprehensive QC testing (such as HCP and so on) and tighter specifications based on the following GENIUS features.
    GENIUS™ Nuclease, premium grade has the same activity and performance with GMP Grade Nuclease, which enables a seamless transition from preclinical development to clinical phases. By using GENIUS™ Nuclease, premium grade in your development phase you can easily transit into clinical and commercial phases and avoid needing to amend your manufacturing process and undertake comparability studies.
    ● Generally usable: Generally usable for removing all forms of DNA and RNA from biological products, reducing viscosity and preventing cell clumping.
    ● Efficient: Efficient reaction, rapid degradation, reduce processing time.
    ● Native: Native structure, tag free, the enzyme activity is more guaranteed.
    ● Ideal: Ideal for DNA and RNA clearance
    ● Ultra pure: Ultra pure: High purity and activit
    ● Specific: Specific nuclease, no protease activity

  • Product Details
    GENIUS™ Nuclease, premium grade is a tag-free recombinant form of Serratia marcescens extracellular endonuclease produced in Escherichia coli using a proprietary process at ACRObiosystems. GMP GENIUS™Nuclease performs optimally at low and physiological salt concentrations (0-200 mM NaCl). The product is a homodimer with monomer molecular masses of about 30 kDa. The enzyme is a non-specific nuclease with high specific activity, degrading single- and double-stranded nucleic acids in any form (single stranded, double stranded, linear, circular, and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5’- phosphorylated oligonucleotides of 3-5 bases in length.
  • Application

    Elimination of nucleic acids from biologics, suitable for samples with 0-200 mM salt in presence of 2-10 mM Mg++
    • viral vaccine development process
    • viral vector development process for cell and gene therapy (CGT)
    • Other biologics development processes

  • Operating Conditions
    GENIUS™ Nuclease, premium grade is functional between pH 6 and 10 (optimal at pH8 - 8.5) , and from 0°C to 42 °C (optimal at 35 °C - 42 °C). Mg2+ (1-2 mM) is required for enzyme activity.1 mM EDTA reduced the activity by 30% in the presence of 1 mM MgCl2; 0.1 M EDTA eliminated all enzyme activity. In the presence of 1 mM MgCl2, enzyme levels were reduced 75% by 0.1 M CaCl2 or 1 M NaCl. Under standard assay conditions, 1 mM iodoacetate had no effect on the enzymatic rate, whereas 1 mM mercaptoethanol and maleic acid reduced the activity by only 5 to 10%. 10 mM p- Chloromercurybenzoate completely inactivates the enzyme, while 0.64 M beta-mercaptoethanol in the presence of 2 M urea causes only partial inactivation of the enzyme. 4 or 7 M Urea increases the enzyme activity.
  • Purity

    >95% as determined by SDS-PAGE.

    >99% as determined by SEC-HPLC.

  • Enzyme Activity
    ≥250 U/μL
  • Host Cell Protein
    <0.05 ng/µg of protein tested by ELISA.
  • Sterility
  • Mycoplasma
  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 20 mM NaCl, 2 mM MgCl2, pH8.0 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt;
    2. -20°C for 24 months under sterile conditions.
Nuclease SDS-PAGE

The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Nuclease SEC-HPLC

The purity of GENIUS™ Nuclease, premium grade (Cat. No. NUE-S5119) was greater than 99% as determined by SEC-HPLC.


Specific activity for GENIUS™ Nuclease, premium grade is measured under standard assay conditions. The specific activity of GENIUS™ Nuclease, premium grade is > 1.2 x 10^6 units/mg protein. One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C, which corresponds approximately to complete digestion of 37 μg DNA. Note that 1 KU=1000 units.

 Nuclease ENZYME

GENIUS™ Nuclease, premium grade shows high specific activity.


The specific activity shows that GENIUS™ Nuclease, premium grade is stable at 4°C for 14 days.


The specific activity shows that GENIUS™ Nuclease, premium grade is stable at 37°C for 48 hours.

Batch Consistency
  • Clinical and Translational Updates

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