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Your Position: Home > Protein > Spike RBD > SPD-C52Hf

SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag

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  • Synonym
    Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD
  • Source
    SARS-CoV-2 S protein RBD (F490L), His Tag(SPD-C52Hf) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (F490L)).
    Predicted N-terminus: Arg 319
  • Molecular Characterization
    Spike RBD Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 26.6 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Spike RBD SDS-PAGE

SARS-CoV-2 S protein RBD (F490L), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 Spike RBD ELISA

Immobilized SARS-CoV-2 S protein RBD (F490L), His Tag (Cat. No. SPD-C52Hf) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-2 ng/mL (QC tested).

 Spike RBD ELISA

Serial dilutions of Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (Cat. No. SPD-M128) were added into SARS-CoV-2 S protein RBD (F490L), His Tag (Cat. No. SPD-C52Hf) and SARS-CoV-2 (COVID-19) S protein RBD, His Tag (Cat.No. SPD-C52H3) : Biotinylated Human ACE2 / ACEH Protein, His,Avitag™ (MALS verified) (Cat.No. AC2-H82E6) binding reactions. The half maximal inhibitory concentrations (IC50) of SARS-CoV-2 S protein RBD (F490L), His Tag (Cat. No. SPD-C52Hf) and SARS-CoV-2 (COVID-19) S protein RBD, His Tag (Cat.No. SPD-C52H3) are 0.20444 μg/mL and 0.78328 μg/mL  respectively (Routinely tested).

Bioactivity-SPR
 Spike RBD SPR

Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD (F490L), His Tag (Cat. No. SPD-C52Hf) with an affinity constant of 53.8 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-BLI
 Spike RBD BLI

Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS-CoV-2 S protein RBD (F490L), His Tag (Cat. No. SPD-C52Hf) with an affinity constant of 57.9 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 Spike RBD BLI

Loaded Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) on AHC Biosensor, can bind SARS-CoV-2 S protein RBD (F490L), His Tag (Cat. No. SPD-C52Hf) with an affinity constant of 45.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates

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