Pseudovirus Neutralization Assay Service

SARS-COV-2 Spike Pseudovirus: A kind of chimeric virus particles formed by replacing the envelope protein in the HIV-1 virus skeleton with SARS-COV-2 Spike. Pseudovirus can simulate the process of SARS-CoV-2 infecting cells. The nucleic acid in the virus particle is artificially designed and modified to make the virus replication-defective and not pathogenic.

As SARS-CoV-2 is highly infectious and pathogenic, so it considerably increases the research difficulty and safety hazards for researchers. Pseudoviruses have the essential components for cell entry and viral infection, but lack of nucleic acid and lose self-replication ability. They have been regarded as the effective virological tools due to their safety and versatility. It is currently widely used in the screening and evaluation of antibodies and antiviral drugs, neutralizing antibody assay, and vaccine efficacy evaluation.As a leading company, ACROBiosystems is committed to accelerating the development of reagent products in the drug research and development field , we also hope to accelerate the development of anti-epidemic drugs through our company's rich technical service experience. ACROBiosystems is now launching the SARS-COV-2 Spike Pseudovirus research-related services and products to help the development of antiviral drugs!

Platform

Principle

Pseudovirus can simulate SARS-CoV-2 to infect target cells

Neutralizer can block pseudovirus from infecting target cells

The SARS-COV-2 Spike Pseudovirus containing the firefly luciferase gene infects target cells and expresses the luciferase gene through its envelope protein—S protein. The S protein is a key component present on the surface of SARS-CoV-2 and is responsible for the viral tropism through host cell recognition by binding to the human angiotensin-converting enzyme 2 (hACE2) receptor. It is the main target for neutralizing antibodies and vaccine design. Neutralizing antibody can block the pseudovirus from infecting host cells. By detecting the decrease in the expression of the luciferase reporter gene, the degree to which the virus is blocked can be inferred to screen or verify the neutralizer.

Process

Service details

Sample type	Neutralizing antibodies, drugs, serum and so on, customers only need to provide samples
Detection reagent	Provide the human ACE2 overexpressing cells, SARS-CoV-2 Spike pseudovirus (wild type and mutant), fluorescence detection reagents, positive antibody control, and other materials needed for the experiment
Detection equipment	PerkinElmer EnSight Microplate Reader
Quality Standard	The ratio of the average fluorescence of the pseudovirus to the average fluorescence of the cell control is larger than 1000, and the background value of the fluorescence of the pseudovirus is less than 1%
Deliverable	Project report
Service period	2-4 weeks

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Case study

Use wild-type (Figures A and B) and Omicron (Figures C and D) S protein pseudovirus to analyze the neutralizing ability of three neutralizing antibodies (SPD-M265, S1N-M122, REGN10987) against SARS-COV-2