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> Pseudovirus Neutralization Antibody Assay Service 
Pseudoviruses are virus particles that have altered nucleic acid sequences within their genome to ensure they are replication-deficient and non-pathogenic. These particles mimic viruses by expressing key proteins on the envelope, which is a key factor in defining a virus’ infectious characteristics. However, the gene modifications enable them to be biologically safe to handle in most laboratories. As such, pseudoviruses are designed to be a substitute for live viruses when evaluating neutralizing antibody production and viral entry inhibition. There are several advantages when using pseudoviruses: Safe, Effective & Flexible; No BSL-3/4 Laboratories Required; High throughput & Quantitative studies.
Screening & Evaluation of antibodies and other antivirals
Neutralizing Antibody Assays
Vaccine Efficacy assessments for the development of vaccines, diagnostics, and therapeutics
| Pseudovirus Type | Associated Viral Disease | Subtype / Serotype |
|---|---|---|
 HPV | Multiple benign tumours or warts, cancer | 6, 11, 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68 |
 RABV | Rabies | CVS-11 |
 SARS-CoV-2 | Novel coronavirus pneumonia | WT, D614G, Alpha, Beta, Gamma, Delta, Delta plus, Lambda, Mu, BA.1, BA.2, BA.2.12.1, BA.2.75, BA.3, BA.4 & BA.5, BF.7, BQ.1.1, XBB.1, XBB.1.5 |
SARS-CoV | Severe Acute Respiratory Syndrome | WT |
MERS-CoV | Middle East Respiratory Syndrome (MERS) | WT |
 Nipah virus | Nipah Viral Encephalitis | MY |
 Ebola virus | Ebola virus disease (EVD) | Zaire |
 SFTSV | Fever with thrombocytopenia syndrome | HB29 |
 Influenza virus | Influenza (flu) | H1N1 |
 VSV | Blistering Stomatitis | Indiana |
| Sample Type | Neutralizing antibodies, drugs, serum samples, etc. are all acceptable. |
| Detection Reagent | ACROBiosystems provides the other materials needed! This includes target cells, pseudoviruses, luciferase detection reagents, etc. |
| Detection Instrument | PerkinElmer EnSight Microplate Reader |
| Quality Standard | 1. Average pseudovirus luciferase intensity compared to average cell control luciferase must be larger than 1000. 2. Background intensity of pseudovirus luciferase is less than 3%. |
| Deliverables | Project report |
| Service Period | 2-4 weeks |
High stability: The method has high stability and the results are true and reliable.
High specificity and sensitivity: High-quality pseudovirus, high-sensitivity detection equipment recognized by the industry.
High-quality detection model： Highly simulating the physiological infection mechanism, which can simulate the actual treatment effect to the greatest extent.
Efficient and fast :The project team will follow up the project one-on-one, give you feedback on the progress of the project periodically, and deliver the experimental results in time.
Extensive service experience and excellent attitude : Outstanding technical service experience, and customized personalized experimental strategies in close linkage with customers.
ACROBiosystems scientist will respond within 24 hours of submission.
Call us at: +1 800-810-0816 or email at services@acrobiosystems.com for consultation.
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