>Star Staining Fluorescent-labeled Products New Generation Tools for CAR Detection
|BCMA||BCA-HF2H3||FITC-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining|
|CD19||CD9-HF2H3||FITC-Labeled Human CD19 (20-291) Protein, His Tag star staining|
|Mesothelin||MSN-HF2H3||FITC-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining|
|Siglec-2||SI2-HF2H4||FITC-Labeled Human Siglec-2 / CD22 Protein, His Tag star staining|
|FMC63||FM3-FY57P1||FITC-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) star staining|
FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H3) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H3) was more than 90% and the molecular weight of this protein is around 24-34 kDa verified by SEC-MALS.
Binding activity of FITC-Labeled Human BCMA and CD19 protein from two different vendors were evaluated by the FACS analysis. The result showed that ACRO's Star Staining FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) and CD19 (Cat. No. CD9-HF2H3) protein have a much higher binding activity than that of the other competitor.
Non-specific binding to non-transduced PBMCs between FITC-Labeled Human BCMA Protein of Acro and competitor. 5e5 of non-transduced PBMCs were stained with FITC-Labeled Human BCMA Protein and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in non-transduced PBMCs, and FITC signal was used to evaluate the non-specific binding activity to non-transduced PBMCs.
Binding activity of the Human BCMA before and after FITC labeling was evaluated in the above FACS analysis. The result shows that FITC-Labeled BCMA (Cat. No. BCA-HF2H3) and unconjugated Human BCMA have almost the same level of binding activity.
Binding affinity of the Human BCMA before and after FITC labeling was evaluated in the above SPR analysis (Biacore T200). The result shows that FITC-Labeled (Cat. No. BCA-HF2H3) and unconjugated Human BCMA, His Tag have almost the same level of affinity.
Binding activity of two different size(left) or three different lots(right) of FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) against anti-BCMA CAR-293 cells was evaluated by flow cytometry. The result shows very high batch-to-batch consistency.
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